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evagreen dye  (Biotium)


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    Structured Review

    Biotium evagreen dye
    Evagreen Dye, supplied by Biotium, used in various techniques. Bioz Stars score: 97/100, based on 3229 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/evagreen+dye/EvaGreen/bio_rxiv__64898__2026__05__09__724006-193-8-10
    Average 97 stars, based on 3229 article reviews
    evagreen dye - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: LNP-based delivery of a Toll-like receptor 9 agonist elicits potent adjuvant effects and antitumor immunity.
    Article Snippet: One microgram of total RNA was reverse-transcribed using the TransScript® First-Strand cDNA Synthesis SuperMix kit (TransGen Biotech). .. The resulting cDNA was used for quantitative real-time PCR (qRT-PCR) with EvaGreen® dye (20× in water; Biotium). ..

    Article Title: Pancreatic Islet Cell Crosstalk: Insight Into α‐/β‐Cell Compensatory Mechanisms
    Article Snippet: Images were acquired on a Leica SP8 microscope, and islet cross‐sectional area was quantified using ImageJ (Schneider et al. ). .. RNA from islets or INS1 cells was isolated using the RNeasy kit (Qiagen, Germany) and reverse‐transcribed (TATAA Biocenter, Sweden). qPCR was performed using EvaGreen dye (Biotium, USA), and relative expression was calculated by the 2 −ΔΔ Ct method using a combination of Rplp0 , Ppia , Hprt , and Ywhaz (mouse) or Ywhaz (INS1) as reference genes validated by NormFinder/geNorm (Figure ). ..

    Article Title: Genomic imprinting of the metabolic regulator gene Klf14 is regulated by a paternal sub-TAD anchored at Mest and a shared enhancer in mice
    Article Snippet: .. The RT-qPCR experiments were performed in triplicate using EvaGreen dye (Biotium), and Tsg (BioBasic) on a QuantStudio 3 Real-Time PCR system (Thermo Fisher Scientific). ..

    Isolation:

    Article Title: Pancreatic Islet Cell Crosstalk: Insight Into α‐/β‐Cell Compensatory Mechanisms
    Article Snippet: Images were acquired on a Leica SP8 microscope, and islet cross‐sectional area was quantified using ImageJ (Schneider et al. ). .. RNA from islets or INS1 cells was isolated using the RNeasy kit (Qiagen, Germany) and reverse‐transcribed (TATAA Biocenter, Sweden). qPCR was performed using EvaGreen dye (Biotium, USA), and relative expression was calculated by the 2 −ΔΔ Ct method using a combination of Rplp0 , Ppia , Hprt , and Ywhaz (mouse) or Ywhaz (INS1) as reference genes validated by NormFinder/geNorm (Figure ). ..

    Reverse Transcription:

    Article Title: Pancreatic Islet Cell Crosstalk: Insight Into α‐/β‐Cell Compensatory Mechanisms
    Article Snippet: Images were acquired on a Leica SP8 microscope, and islet cross‐sectional area was quantified using ImageJ (Schneider et al. ). .. RNA from islets or INS1 cells was isolated using the RNeasy kit (Qiagen, Germany) and reverse‐transcribed (TATAA Biocenter, Sweden). qPCR was performed using EvaGreen dye (Biotium, USA), and relative expression was calculated by the 2 −ΔΔ Ct method using a combination of Rplp0 , Ppia , Hprt , and Ywhaz (mouse) or Ywhaz (INS1) as reference genes validated by NormFinder/geNorm (Figure ). ..

    Expressing:

    Article Title: Pancreatic Islet Cell Crosstalk: Insight Into α‐/β‐Cell Compensatory Mechanisms
    Article Snippet: Images were acquired on a Leica SP8 microscope, and islet cross‐sectional area was quantified using ImageJ (Schneider et al. ). .. RNA from islets or INS1 cells was isolated using the RNeasy kit (Qiagen, Germany) and reverse‐transcribed (TATAA Biocenter, Sweden). qPCR was performed using EvaGreen dye (Biotium, USA), and relative expression was calculated by the 2 −ΔΔ Ct method using a combination of Rplp0 , Ppia , Hprt , and Ywhaz (mouse) or Ywhaz (INS1) as reference genes validated by NormFinder/geNorm (Figure ). ..

    Polymerase Chain Reaction:

    Article Title: Mutation of Tubgcp6 induces hematopoietic stem and progenitor cell exhaustion in zebrafish.
    Article Snippet: .. The tubgcp6, tubgcp3 and tubgcp5 mutants were genotyped by PCR followed by high-resolution melting (HRM) analysis with EvaGreen Dye (17E0331-1056166, Biotium) on a LightCycler 96 system (Roche). ..

    Article Title: Phosphoserine aminotransferase SerC is a central metabolic checkpoint and druggable vulnerability in Mycobacterium tuberculosis
    Article Snippet: .. For these reactions a 10 μl PCR was set up that consisted of 5 μl of Q5 High-Fidelity 2× Master Mix (NEB, UK), 0.5 μl × 20 EvaGreen® Dye (Biotium), 0.5 μl IS6 and 0.5 μl of Himar-1 MycoMarT7 mariner transposon (2 pmol μl -1 primers), 1 μl of template DNA and 2.5 μl of nuclease free water. ..

    Quantitative RT-PCR:

    Article Title: Genomic imprinting of the metabolic regulator gene Klf14 is regulated by a paternal sub-TAD anchored at Mest and a shared enhancer in mice
    Article Snippet: .. The RT-qPCR experiments were performed in triplicate using EvaGreen dye (Biotium), and Tsg (BioBasic) on a QuantStudio 3 Real-Time PCR system (Thermo Fisher Scientific). ..

    other:

    Article Title: Spatial Organization and Detection of Social Odors in Mouse Primary Olfactory System
    Article Snippet: Triton-X , Sigma , Cat# T8787.

    Article Title: Droplet-compatible single-cell DNA methylation sequencing with PreTIC
    Article Snippet: PFA fixed human (GM12878) and mouse (A20) cell lines were thawed from -80 °C and mixed in an approximate ratio of 4:1, and proceeded with library preparation as described above, except that prior to the final TTB wash and 10x submission, the cells were resuspended in TTB supplemented with 1.25 mM EvaGreen® Dye (Biotium 31000), and subjected to flow sorting.



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